منابع مشابه
Genetic modification of chicken germ cells
Over the past two decades numerous reports have demonstrated that the genetic modification of poultry genomes has great potential for improving poultry production; moreover, it may be used as a powerful tool for the production of industrial proteins. To date, transgenic techniques have been established for generating transgenic birds that express recombinant human proteins in hen eggs, as well ...
متن کاملEfficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons.
The derivation of germ-line competent avian primordial germ cells establishes a cell-based model system for the investigation of germ cell differentiation and the production of genetically modified animals. Current methods to modify primordial germ cells using DNA or retroviral vectors are inefficient and prone to epigenetic silencing. Here, we validate the use of transposable elements for the ...
متن کاملisolation, culture and characterization of chicken primordial germ cells
nowadays, production of recombinant proteins in eukaryotes is gaining good deal of attention. transgenic chicken as a eukaryotic system has a high potential for producing recombinant proteins. post-translational changes, especially glycosylation, are characteristic of the eukaryotic proteins. in practice we need to choose a proper expressing host when considering over-expression of a recombinan...
متن کاملIsolation, Culture and Characterization of Chicken Primordial Germ Cells
Nowadays, production of recombinant proteins in eukaryotes is gaining good deal of attention. Transgenic chicken as a eukaryotic system has a high potential for producing recombinant proteins. Post-translational changes, especially glycosylation, are characteristic of the eukaryotic proteins. In practice we need to choose a proper expressing host when considering over-expression of a recombinan...
متن کاملCryopreservation of specialized chicken lines using cultured primordial germ cells
Biosecurity and sustainability in poultry production requires reliable germplasm conservation. Germplasm conservation in poultry is more challenging in comparison to other livestock species. Embryo cryopreservation is not feasible for egg-laying animals, and chicken semen conservation has variable success for different chicken breeds. A potential solution is the cryopreservation of the committe...
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ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 2012
ISSN: 0077-8923,1749-6632
DOI: 10.1111/j.1749-6632.2012.06744.x